Takara Tetracycline-inducible expression: Tet-Off cell lines

Tet-Off cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by removing doxycycline (Dox) from the culture medium. Tet-On 3G cell lines, which are our highest-performing pre-made cell lines for this system, are located on a separate page. Tet-On 3G cell lines are activated by removing Dox from the culture medium.

Overview

• Tet-On 3G cell lines, our highest-performing pre-made cell lines for the Tet system, are located on a separate page
• Stably expressed Tet-Off transactivator protein in commonly used cell lines.
• Induce expression of your gene of interest by removing doxycycline (Dox) from the culture of transfected cells
• Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g., PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

More Information

Applications

• Inducible gene expression in mammalian cells

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.