Takara Definitive endoderm to hepatocyte differentiation kit

The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.

The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.

There are several options for source materials to generate functional hepatocytes: your own DE cells, our DE cells, or DE cells you generate using the Cellartis Definitive Endoderm Differentiation Kit. This kit has been successfully tested with DE cells derived from 28 different human pluripotent cell lines. Our cells, Cellartis Definitive Endoderm Cells (from ChiPSC18), are suitable as the starting material or as a positive control. If you desire a customized (standard, patient-specific, or disease-specific) genetic background for your DE cells, use the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. # Y30055).

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Overview

• Complete kit—includes four different media sets and a ready-to-use coating, sufficient for a 23-day protocol for differentiating definitive endoderm (DE) cells into hepatocytes
• Robust protocol—tested with more than 25 different stem cell lines
• Efficient differentiation—yields >90% pure hepatocytes

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Components

• Cellartis Hepatocyte Differentiation Kit (Cat. # Y30050)
  • Hepatocyte Thawing and Seeding Medium, 1 bottle (25 ml)
  • Hepatocyte Progenitor Medium, 1 bottle (50 ml)
  • Hepatocyte Maturation Medium Base, 2 bottles (24 ml each)
  • Hepatocyte Maturation Medium Supplement, 1 tube (2.6 ml)
  • Hepatocyte Maintenance Medium, 3 bottles (50 ml each)
  • Hepatocyte Coating, 1 tube (7.5 ml)

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.