Thermo Fisher Scientific GLI1 Monoclonal Antibody (UMAB170), UltraMAB
GLI1 단백질을 인식하는 Mouse monoclonal antibody (UMAB170). Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합. Human 시료 반응성, Lyophilized 형태로 제공. PBS buffer에 8% trehalose 포함, -20°C 보관 권장. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB170 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–410 of human GLI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. Its activity and nuclear localization are negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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