Thermo Fisher Scientific eIF3e Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03-0.1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (KLNQNSRSEAPN) corresponding to the C-terminus amino acids of EIF3E (aa 425-437). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF3e,uniProtId:P60228-1,ncbiNodeId:9606,antigenRange:425-437,antigenLength:445,antigenImageFileName:PA5-19411_eIF3e_P60228-1_Goat.svg,antigenImageFileNamePDP:PA5-19411_eIF3e_P60228-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10984437

Product Specific Information

This antibody is predicted to react with bovine, canine and porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.

Target Information

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.