
Thermo Fisher Scientific Orf2 Polyclonal Antibody
Mouse ORF2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 ELISA에 최적화. Affinity chromatography로 정제된 고순도 항체. -20°C에서 보관하며 동결-해동 반복을 피해야 함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Orf2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1.0 µg/mL |
| ELISA | 1.0 µg/µL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of mouse ORF2 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.07 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Orf2 is directed against mouse ORF2 near a C-terminus position. A BLAST analysis suggests reactivity with this protein from human and feline based on 100% homology for the immunogen sequence.
Target Information
The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embryonic development, and mutations of L1 may result in detrimental de novo insertions into the genome, negatively affecting gene expression.
L1 elements are critical components in the mammalian genome, and much remains unknown regarding these proteins. Studies indicate that LINE-1 is activated in fetal oocytes and L1ORF2p may contribute to the eradication or selection of oocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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