Thermo Fisher Scientific H3R3me2a Polyclonal Antibody
H3R3me2a 폴리클로날 항체로 인간 Histone H3.2의 아르기닌 2 비대칭 이메틸화 부위를 인식. WB, IHC, ICC, DB 등 다양한 응용에 적합. 토끼 IgG 기반, 액상 형태로 -20°C 보관. 높은 특이성과 순도를 가진 연구용 항체.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Asym-dimethyl Arg2] affinity purified antibody prepared from rabbit serum immunized with synthetic dimethylated peptide surrounding Arginine 2 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.71 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2. BLAST analysis suggests cross-reactivity with human, mouse, and C. elegans. Predicted to react with rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 600-401-I53_H3R3me2a_P68431-1_Rabbit.svg, 600-401-I53_H3R3me2a_Q71DI3-1_Rabbit.svg, 600-401-I53_H3R3me2a_P68431-1_Rabbit_PDP.jpeg, 600-401-I53_H3R3me2a_Q71DI3-1_Rabbit_PDP.jpeg)
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