Thermo Fisher Scientific VE-cadherin Polyclonal Antibody
VE-cadherin 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB 및 ICC/IF에 적합합니다. 인간 및 마우스 반응성, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용되며 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific VE-cadherin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.1–1 µg/mL | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mammal, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human VE Cadherin (CD144, UniProt ID: P33151-1). Antigen range: 750–784. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979589 |
Product Specific Information
- Heat-mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) is recommended prior to tissue staining.
- Predicted to react with cow and pig based on sequence homology.
- Store antibody at 4ºC for 1–2 weeks; for long-term storage, store at -20ºC.
Target Information
VE-cadherin is a calcium-dependent cell-cell adhesion glycoprotein and a member of the cadherin superfamily. It is located within a six-cadherin cluster on chromosome 16 and is implicated in loss of heterozygosity events in breast and prostate cancer.
VE-cadherin comprises five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It mediates homophilic cell adhesion and plays a key role in endothelial cell cohesion and junction organization.
An alternative splice variant has been described, though the full-length sequence has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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