Thermo Fisher Scientific MRPS27 Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 사용 가능한 인간 MRPS27 단백질 인식 토끼 폴리클로날 항체. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–153 of Human MRPS27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547933 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2, HCT116
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and are involved in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit, with approximately 75% protein to rRNA composition, unlike prokaryotic ribosomes where this ratio is reversed. Prokaryotic ribosomes contain a 5S rRNA, which is absent in mammalian mitoribosomes. The proteins comprising the mitoribosome differ greatly among species, both in sequence and biochemical properties, making sequence homology recognition difficult. The MRPS27 gene encodes a 28S subunit protein that may functionally associate with death-associated protein 3 (DAP3).
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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