Thermo Fisher Scientific Phospho-HGS (Tyr216) Polyclonal Antibody
Phospho-HGS (Tyr216) 특이적 폴리클로날 항체로, 인간·마우스·랫트에서 반응. Western blot과 ELISA에 적합. 항원 친화 크로마토그래피로 정제된 고순도 제품. 세포 내 신호전달 및 단백질 인산화 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human Hrs around the phosphorylation site of Y216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610402 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathways via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin.
HGS may concentrate ubiquitinated receptors within clathrin-coated regions and is involved in down-regulation of receptor tyrosine kinase via multivesicular bodies (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to lysosomal sorting/trafficking processes.
HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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