Thermo Fisher Scientific Human IL-4 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 7 publications 7 publications

Neutralization (Neu)

-

View 3 publications 3 publications

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

T-Cell Activation (TCA)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 660 publications 660 publications

Miscellaneous PubMed (Misc)

-

View 54 publications 54 publications

Product Specifications

Species

Human

Published species

Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat

Expression System

E. coli

Amino acid sequence

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Molecular weight

15.1 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-4 is a 15.1 kDa globular protein containing 130 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.