Thermo Fisher Scientific Peroxidase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:1,000-1:5,000

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:2,000-1:10,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peroxidase (Horseradish)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Peroxidase (Horseradish). Cross-reactivity against Peroxidase from other tissues and species may occur but has not been specifically determined.

Target Information

HRP, also known as horseradish peroxidase, is an enzyme that is commonly used in biochemistry and molecular biology research. It is derived from the root of the horseradish plant and catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays, such as ELISAs and Western blots, due to its high sensitivity and stability. It can also be used as a tracer in enzyme-linked immunosorbent assays (ELISAs) and as a labeling agent in fluorescent microscopy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.