Thermo Fisher Scientific PKN1 Monoclonal Antibody (4H10B1)
PKN1 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 포함. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H10B1 |
| Immunogen | Purified recombinant fragment of human PKN1 (AA: 442–620) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787472 |
Product Specific Information
MA5-31849 has been tested in indirect ELISA.
Target Information
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. It can also be activated by phospholipids. PDPK1 (PDK1) phosphorylates this kinase, which may mediate insulin signals to the actin cytoskeleton. Proteolytic activation by caspase-3 or related proteases during apoptosis suggests its role in apoptotic signal transduction. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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