Thermo Fisher Scientific CD20 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 55 publications 55 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:300

View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Cat, Human, Mouse

Published species

Bovine, Cat, Dog, Horse, Human, Mink, Mouse, Mustelid, Non-human primate, Pig, Rat, Rhesus monkey, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human CD20 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD20,uniProtId:P11836-1,ncbiNodeId:9606,antigenRange:297,antigenLength:297,antigenImageFileName:PA5-16701_CD20_P11836-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16701_CD20_P11836-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.077 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10980806

Product Specific Information

Upon delivery aliquot and store at -20ºC, avoiding freeze/thaw cycles.

For IHC staining, antigen retrieval is not essential but may optimize staining. Recommended positive controls: BJAB WCL and tonsil.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.