Thermo Fisher Scientific CD20 Polyclonal Antibody
CD20 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용 가능. 인간 및 여러 종에 반응하며, 항원 친화 크로마토그래피로 정제됨. 연구용으로 B세포 표지 및 면역학 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 55 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300 | View 10 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 5 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human, Mouse |
| Published Species | Bovine, Cat, Dog, Horse, Human, Mink, Mouse, Mustelid, Non-human primate, Pig, Rat, Rhesus monkey, Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human CD20 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.077 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| RRID | AB_10980806 |
Product Specific Information
Upon delivery, aliquot and store at -20ºC, avoiding freeze/thaw cycles.
For IHC staining, antigen retrieval is not essential but may optimize staining.
Recommended positive controls: BJAB WCL and tonsil.
Target Information
CD20 is a non-glycosylated surface phosphoprotein with a molecular weight of 33–37 kDa depending on phosphorylation. It is expressed on mature and most malignant B cells, as well as some T lymphocytes and follicular dendritic cells. CD20 regulates calcium conductance, cell cycle progression, and B-cell proliferation. It is associated with lipid rafts, and its expression makes it a key target for antibody-mediated B-cell depletion therapies.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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