Thermo Fisher Scientific Quantifiler HP DNA Quantification Kit

Applied Biosystems™ Quantifiler™ HP DNA Quantification Kit

The Quantifiler HP (Human Plus) DNA Quantification Kit is a robust, highly sensitive real-time PCR kit that enables forensic laboratories to obtain both quantitative and qualitative assessments of total human DNA in a single reaction. This facilitates optimal STR analysis selection (autosomal or miniSTR) and improves downstream analysis success rates. The kit works seamlessly with HID Real Time PCR Analysis Software v1.2 and the Applied Biosystems 7500 Real-Time PCR System.

주요 특징

Highly Sensitive

• Detection limit: <1 pg/μL
• Quantifies DNA concentrations from 0.005 ng/μL to >50 ng/μL
• Flexible protocols for higher concentration samples

Highly Specific

• Detects human and higher primate DNA

Highly Informative

• Suitable for DNA from buccal swabs, blood, semen, tissue, and other human sources
• Guides selection of optimal AmpFLSTR PCR Amplification Kit (autosomal or miniSTR)
• Quality Index improves prediction of STR amplification performance, especially in degraded samples
• Detects PCR inhibitors, guiding purification or miniSTR kit selection (MiniFiler, NGM SElect, GlobalFiler)

Highly Reliable

• Utilizes gold-standard TaqMan real-time PCR technology
• Provides accurate quantification across a wide dynamic range

Highly Efficient

• Minimizes sample consumption and streamlines workflow
• Results available in approximately 1 hour
• Fully optimized with Applied Biosystems 7500 Real-Time PCR System and HID Real Time PCR Analysis Software v1.2

A Highly Informative Workflow Tool

Optimized and validated as part of a complete DNA testing system with AmpFLSTR PCR Amplification kits. Compatible with common extraction technologies for easy integration into existing lab workflows. Fully automatable with most liquid handling robotic platforms.

Comparison to Quantifiler Human Kits

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