Thermo Fisher Scientific EpCAM/CD326 (Epithelial Marker) Monoclonal Antibody (VU-1D9)
EpCAM/CD326 단백질을 인식하는 마우스 단클론 항체로, 인간 유래 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 단백질 A/G로 정제된 액상 형태이며, 보존제 없이 PBS(pH 7.4)에 담겨 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| Not applicable (N/A) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VU-1D9 |
| Immunogen | Small cell lung carcinoma cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
- Does not react with Rat or Ferret.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a calcium-independent cell adhesion molecule and regulates cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, influencing epithelial differentiation and growth.
Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial lineage.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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