Thermo Fisher Scientific CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (128)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:250–1:2,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human RANKL/OPGL/TNFSF11/CD254.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family, a ligand for osteoprotegerin and a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses.

T cell activation induces expression of this gene, leading to increased osteoclastogenesis and bone loss. It activates antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.

Targeted disruption of the related gene in mice caused severe osteopetrosis and absence of osteoclasts. Deficient mice showed defects in early T and B lymphocyte differentiation and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.