Thermo Fisher Scientific MUC1/CA15-3/EMA/CD227 Monoclonal Antibody (MUC1, 967)
MUC1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. Human 시료 반응성이 있으며, Protein A/G 정제 및 PBS buffer에 보존됩니다. 암 연구 및 세포막 단백질 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MUC1, 967 |
| Immunogen | Human milk-fat globule membranes (HMFGM) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed), incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
MUC1 (Mucin 1, also known as Episialin, MAM-6, CA 15-3, PEM, and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic lineages. It is a transmembrane glycoprotein with a large extracellular domain containing multiple tandem repeats that vary between alleles (30–90 repeats), resulting in size heterogeneity.
MUC1 is highly expressed in more than 90% of breast carcinomas and metastases. It associates with all four erbB receptors and localizes with EGFR in lactating glands. Overexpression and aberrant glycosylation of MUC1 are associated with various carcinomas. Multiple alternatively spliced variants have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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