Thermo Fisher Scientific CD31 Monoclonal Antibody (TLD-3A12)
CD31(PECAM-1)을 인식하는 Mouse monoclonal antibody로, Rat와 Rhesus monkey 시료에 반응합니다. WB, IHC, Flow Cytometry 등 다양한 응용에 적합하며, Protein A로 정제된 1 mg/mL 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:100 | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 2 publications |
| Flow Cytometry (Flow) | 1:10–1:100 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rhesus monkey, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLD-3A12 |
| Immunogen | Activated, Lewis rat derived microglial cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928154 |
Available Conjugated Formats
Product Specific Information
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type I transmembrane protein expressed primarily on endothelial cells, platelets, and leukocytes.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through dual immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is expressed ubiquitously within the vascular compartment, mainly at junctions between adjacent cells. The N-terminal Ig-like domain mediates homophilic binding, critical for cell-cell interactions.
It plays diverse roles in:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis
- Apoptosis regulation
- Immunoreceptor signaling modulation
- Autoimmunity and thrombosis regulation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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