Thermo Fisher Scientific CD31 Monoclonal Antibody (TLD-3A12)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type I transmembrane protein expressed primarily on endothelial cells, platelets, and leukocytes.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through dual immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is expressed ubiquitously within the vascular compartment, mainly at junctions between adjacent cells. The N-terminal Ig-like domain mediates homophilic binding, critical for cell-cell interactions.
It plays diverse roles in:

• Integrin-mediated adhesion
• Transendothelial migration
• Angiogenesis
• Apoptosis regulation
• Immunoreceptor signaling modulation
• Autoimmunity and thrombosis regulation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.