Thermo Fisher Scientific F(ab`)2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

ELISA (ELISA)

1:1,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Murine IgG.

Conjugate

AP AP AP

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Rabbit/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Rabbit/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Rabbit/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Rabbit/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Rabbit/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Rabbit/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Rabbit/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Rabbit/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Rabbit/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Rabbit/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Rabbit/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Rabbit/)
• [DyLight 755](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Rabbit/)
• [DyLight 800](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 8.0, with 1% BSA, 25% glycerol

Contains

<0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931517

Target

IgG

Cross Adsorption

Against Human IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Cross reactivity is observed with rat IgG. Cross-reactivity with human IgG has been minimized by solid phase absorption. F(ab`)2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.

Rabbit anti Mouse IgG antibody recognizes mouse IgG.Cross reactivity is observed with rat IgG. Cross-reactivity with human IgG has been minimised by solid phase absorption.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.