Thermo Fisher Scientific NCOA4 Polyclonal Antibody
NCOA4 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되어 고순도(>95%) 확보. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 348–398 of human ARA70 (NCOA4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553502 |
Product Specific Information
This antibody detects endogenous NCOA4 protein at a molecular weight of approximately 70 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Androgen receptor (AR) coactivator ARA70, also designated NCOA4, RFG, and ELE1, is a putative co-activator that enhances AR activity. In human thyroid carcinomas, the Ret proto-oncogene fuses with ARA70 to form Ret/PTC3 through intrachromosomal inversion.
ARA70 exists as two isoforms: ARA70a and ARA70b.
- ARA70a: Widely expressed, highest in testis and adipose tissues.
- ARA70b: Expressed only in testis.
ARA70a functions as a ligand-enhanced co-activator of PPARγ in adipocytes. ARA70 may be involved in prostate and ovarian carcinogenesis and may mediate estrogen-androgen synergism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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