Thermo Fisher Scientific Macrophages (Mature) Monoclonal Antibody (25F9), FITC
성숙 대식세포 특이 단클론 항체로 인간 시료에 반응하며 FITC 형광 표지로 세포 분석에 적합. 다양한 조직의 대식세포 검출에 활용 가능. Protein G 정제, 4°C 암소 보관.
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Thermo Fisher Scientific Macrophages (Mature) Monoclonal Antibody (25F9), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25F9 |
| Immunogen | Recognizes a protein of 86 kD on the surface and cytoplasm of mature macrophages |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_3090514 |
Available Formats
- Unconjugated
- Biotin
- Custom conjugation available upon request
Product Specific Information
The antibody is associated with fully differentiated tissue macrophages in both normal and diseased tissues, independent of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells but appears after 6–7 days of monocyte culture. Some melanoma and carcinoma cell lines are also positive. The monoclonal antibody 25F9 cross-reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages, and Kupffer cells.
Target Information
Macrophages are specialized white blood cells responsible for detecting, engulfing, and destroying foreign substances, dead cells, and debris. Mature macrophages are distributed throughout various tissues, performing location-specific functions:
- Alveolar macrophages: Found in lungs; remove inhaled particles.
- Kupffer cells: Found in liver; remove old or damaged red blood cells.
Understanding macrophage function is essential for developing treatments for cancer, autoimmune disorders, and infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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