Thermo Fisher Scientific GTF2E1 Recombinant Rabbit Monoclonal Antibody (23GB6310)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Flow Cytometry (Flow)

1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

23GB6310

Immunogen

A synthesized peptide derived from human TFIIE alpha (400-439AA). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GTF2E1,uniProtId:P29083-1,ncbiNodeId:9606,antigenRange:400-439,antigenLength:439,antigenImageFileName:MA5-53018_GTF2E1_P29083-1_Rabbit.svg,antigenImageFileNamePDP:MA5-53018_GTF2E1_P29083-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and 34, 000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.