Thermo Fisher Scientific CD10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/ml

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sf 21-derived recombinant mouse Neprilysin/CD10 Tyr52-Trp750 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD10,uniProtId:Q61391-1,ncbiNodeId:10090,antigenRange:52-750,antigenLength:750,antigenImageFileName:PA5-47075_CD10_Q61391-1_Goat.svg,antigenImageFileNamePDP:PA5-47075_CD10_Q61391-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607390

Product Specific Information

In sandwich ELISAs, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitts, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5 untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.