Thermo Fisher Scientific SIGLEC7 Polyclonal Antibody
Human SIGLEC7 단백질을 인식하는 Goat Polyclonal Antibody로, WB, IHC, Flow Cytometry, Neutralization에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간 시료에 반응하며, 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
| Neutralization (Neu) | 0.3–0.9 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Siglec-7/CD328 (Gln19–Gly357) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576436 |
Product Specific Information
- In direct ELISAs, approximately 15% cross-reactivity with recombinant human Siglec-9 is observed.
- Approximately 5% cross-reactivity with recombinant mouse Siglec-E, rhSiglec-6, and rhSiglec-8 is observed.
- Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
Siglec-7 is a putative adhesion molecule that mediates sialic acid–dependent binding to cells, preferentially recognizing α2,3- and α2,6-linked sialic acids. It also binds disialogangliosides such as disialogalactosyl globoside and disialyl lactotetraosylceramide.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
In immune response, Siglec-7 may act as an inhibitory receptor, recruiting cytoplasmic phosphatases via SH2 domains to block signal transduction and inhibit NK cell cytotoxicity.
It may also play a role in hematopoiesis, inhibiting differentiation of CD34⁺ precursors toward the myelomonocytic lineage and proliferation of leukemic myeloid cells in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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