Thermo Fisher Scientific HLA-C Monoclonal Antibody (3H3A5)
인간 HLA-C 단백질을 인식하는 Mouse IgG1 단클론 항체로 다양한 면역분석(WB, IHC, ICC/IF, Flow, ELISA)에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도의 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific HLA-C Monoclonal Antibody (3H3A5)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H3A5 |
| Immunogen | Purified recombinant fragment of human HLA-C (AA: 25–308) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3090840 |
Target Information
HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is membrane-anchored and plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen.
HLA-C molecules are expressed in nearly all cells. The heavy chain (~45 kDa) gene contains 8 exons:
- Exon 1 encodes the leader peptide
- Exons 2 and 3 encode the α1 and α2 domains (peptide-binding regions)
- Exon 4 encodes the α3 domain
- Exon 5 encodes the transmembrane region
- Exons 6 and 7 encode the cytoplasmic tail
Polymorphisms within exon 2 and exon 3 determine peptide-binding specificity. Typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.
HLA and MHC antibodies are essential in immunopeptidomics, aiding identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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