Thermo Fisher Scientific HLA-F Monoclonal Antibody (3F11F4)
HLA-F 단백질을 인식하는 mouse monoclonal antibody (clone 3F11F4). Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. Protein G 정제, PBS buffer에 0.05% sodium azide 포함. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | 1:200–1:400 | |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F11F4 |
| Immunogen | Purified recombinant fragment of human HLA-F (AA: 22–305) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090523 |
Target Information
HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove and is thought to bind a restricted subset of peptides for immune presentation.
HLA and MHC antibodies play a significant role in immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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