Thermo Fisher Scientific Phospho-ATP Citrate Lyase (Ser455, Ser457) Polyclonal Antibody
ATP Citrate Lyase의 Ser455/457 인산화 형태를 인식하는 Rabbit Polyclonal Antibody. Western blot에 적합하며, 인산화된 내인성 단백질 검출 가능. Human, Mouse, Rat 등 다양한 종에 반응. PBS/glycerol buffer 형태로 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ACLY (Accession P53396), corresponding to amino acid residues around phosphorylated Ser457 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816585 |
Product Specific Information
This antibody detects endogenous levels of ATP citrate lyase only when phosphorylated at Ser455/457.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues.
The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits.
It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate.
The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.
In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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