
Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI5E4), TrueMAB
인간 Ki-67 단백질을 인식하는 Mouse monoclonal antibody로, 세포 증식 마커 연구에 적합합니다. IHC(P)에서 1:100 희석으로 사용되며, 무보존제 동결건조 형태로 제공됩니다. 재구성 후 1 mg/mL 농도로 사용 가능합니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160–1493 of human MKI67 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and is distributed in a cell cycle-dependent manner—perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis. It aids in determining the growth fraction of neoplasms, correlating with tumor grade and prognosis. Commonly used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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