Thermo Fisher Scientific HSPB11 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human HSPB11. Recombinant protein control fragment (Product #RP-94032). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSPB11,uniProtId:Q9Y547-1,ncbiNodeId:9606,antigenRange:4-71,antigenLength:144,antigenImageFileName:PA5-56362_HSPB11_Q9Y547-1_Rabbit.svg,antigenImageFileNamePDP:PA5-56362_HSPB11_Q9Y547-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2642579

Product Specific Information

Immunogen sequence: IDLCLSSEGS EVILATSSDE KHPPENIIDG NPETFWTTTG MFPQEFIICF HKHVRIERLV IQSYFVQT

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.

Target Information

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.