Thermo Fisher Scientific alpha Amylase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:500-1:5,000

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

a-Amylase [Bacillus amyloliquefaciens]

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against a-Amylase from other tissues and species may occur but have not been specifically determined.

Target Information

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.