Thermo Fisher Scientific alpha Amylase Polyclonal Antibody
Bacillus amyloliquefaciens 유래 α-Amylase를 인식하는 Rabbit Polyclonal Antibody. WB, ELISA, IP 등 다양한 응용에 적합. 1 mg/mL 농도의 액상 제품으로 -20°C 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific alpha Amylase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | α-Amylase (Bacillus amyloliquefaciens) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified α-Amylase (Bacillus amyloliquefaciens). Cross-reactivity against α-Amylase from other tissues and species may occur but has not been specifically determined.
Target Information
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. The human genome contains several amylase genes expressed at high levels in salivary glands or pancreas. It is an amylase isoenzyme produced by the salivary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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