Thermo Fisher Scientific DAAM1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DAAM1,uniProtId:Q9Y4D1-1,ncbiNodeId:9606,antigenRange:769-1068,antigenLength:1078,antigenImageFileName:PA5-109614_DAAM1_Q9Y4D1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109614_DAAM1_Q9Y4D1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855025

Product Specific Information

Immunogen sequence: FKKKFAERVA EVKPKVEAIR SGSEEVFRSG ALKQLLEVVL AFGNYMNKGQ RGNAYGFKIS SLNKIADTKS SIDKNITLLH YLITIVENKY PSVLNLNEEL RDIPQAAKVN MTELDKEIST LRSGLKAVET ELEYQKSQPP QPGDKFVSVV SQFITVASFS FSDVEDLLAE AKDLFTKAVK HFGEEAGKIQ PDEFFGIFDQ FLQAVSEAKQ ENENMRKKKE EEERRARMEA QLKEQRERER KMRKAKENSE ESGEFDDLVS ALRSGEVFDK DLSKLKRNRK RITNQMTDSS RERPITKLNF

Target Information

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.