Thermo Fisher Scientific Cdc20 Polyclonal Antibody
Cdc20 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성을 가지며, Affinity Chromatography로 정제되었습니다. PBS/glycerol buffer에 보관되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–100 of human CDC20 (NP_0012462) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.172 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806139 |
Product Specific Information
- Positive Samples: LO2, HT-29, A-431
- Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Immunogen Sequence:
MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ
Target Information
CDC20 is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. It is regulated by MAD2L1: in metaphase, the MAD2L1-CDC20-APC/C ternary complex is inactive, while in anaphase, the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates synaptic vesicle clustering at the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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