Thermo Fisher Scientific TGF alpha Polyclonal Antibody
TGF alpha 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. 인간 및 마우스 시료에 반응하며, 세포막 및 분비 단백질 검출에 사용됩니다. 고순도 친화 크로마토그래피 정제, -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24–98 of human TGFA (NP_001093161.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913616 |
Product Specific Information
- Positive test controls: 293T, Jurkat, Mouse lung
- Subcellular localization: Cell membrane, Secreted, Single-pass type I membrane protein, extracellular space
- Immunogen sequence:
ENSTSPLSDP PVAAAVVSHF NDCPDSHTQF CFHGTCRFLV QEDKPACVCH SGYVGARCEH ADLLAVVAAS QKKQA
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates proliferation of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) has 33% homology to EGF, binds EGFR, activates tyrosine phosphorylation, and promotes cell proliferation. It plays a role in tumor initiation by inducing a reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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