Thermo Fisher Scientific Phospho-CaMKII alpha/beta/delta (Ser235) Polyclonal Antibody
CaMKII alpha/beta/delta 단백질의 Ser235 인산화 상태를 특이적으로 검출하는 폴리클로날 항체. WB, IHC, ICC/IF 등 다양한 응용에 적합. Rabbit IgG 기반, 고순도 정제 및 안정적 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CAMK2B (Accession Q13554), corresponding to amino acid residues around phosphorylated Ser235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817069 |
Product Specific Information
Antibody detects endogenous levels of CaMKII only when phosphorylated at Ser235.
Target Information
The Ca²⁺/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII, and CaMKIV.
CaMKII is an ubiquitously expressed serine/threonine protein kinase activated by Ca²⁺ and calmodulin (CaM), and is involved in regulation of the cell cycle and transcription.
There are four CaMKII isozymes—alpha, beta, gamma, and delta—which may or may not be co-expressed in the same tissue type.
CaMKII alpha is autophosphorylated on Thr286 upon binding of the Ca²⁺/CaM complex to its autoinhibitory domain, a process known as Ca²⁺/CaM trapping, thought to be involved in synaptic encoding of information.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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