Thermo Fisher Scientific MADD Polyclonal Antibody
MADD 단백질을 표적으로 하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB 및 IP에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인간 시료 반응성이 높습니다. 4°C에서 보관하며 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1100 and 1150 of human MAP-kinase activating death domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Tumor necrosis factor receptor 1 (TNFR1) contains a death receptor domain that mediates interactions involved in downstream signaling of TNF.
A novel protein, MADD (mitogen activated protein (MAP)-kinase activating death domain protein) associates with the death domain of TNFR1 through its own C-terminal death domain. This interaction is critical for TNFR1 signal generation.
Overexpression of MADD activates MAP kinases ERK and JNK and induces phosphorylation of cytosolic phospholipase A2, linking TNF signaling to MAP kinase activation and arachidonic acid release.
MADD shows 98% homology with DENN (differentially expressed in neoplasmic vs. normal cells) and 93% sequence conservation with Rab3, a GDP/GTP exchange protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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