Thermo Fisher Scientific MADD Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Tumor necrosis factor receptor 1 (TNFR1) contains a death receptor domain that mediates interactions involved in downstream signaling of TNF.
A novel protein, MADD (mitogen activated protein (MAP)-kinase activating death domain protein), associates with the death domain of TNFR1 through its own C-terminal death domain, which is critical for TNFR1 signal generation.

Overexpression of MADD activates MAP kinases (ERK and JNK) and induces phosphorylation of cytosolic phospholipase A2, suggesting MADD links TNF signaling to MAP kinase activation and arachidonic acid release.
MADD shares 98% homology with DENN (differentially expressed in neoplasmic vs. normal cells), a JNK3 substrate involved in hypoxia and stress-induced signaling.
It also shows 93% sequence conservation with Rab3 GDP/GTP exchange protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.