Thermo Fisher Scientific TWIST1 Polyclonal Antibody
TWIST1 단백질을 인식하는 Thermo Fisher Scientific의 sheep polyclonal antibody로, 인간 시료에 반응합니다. ICC/IF 및 Flow Cytometry에 적합하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. Lyophilized 형태로 제공되며, -20°C 보관이 권장됩니다.
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Thermo Fisher Scientific TWIST1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human Twist-1 (Met1–His202) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607737 |
Product Specific Information
- In direct ELISAs, less than 16% cross-reactivity with recombinant human Twist-2 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogenesis by sequestering E proteins, inhibiting trans-activation by MEF2, and blocking DNA-binding by MYOD1 through physical interaction.
TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B, and regulates cranial suture patterning and fusion.
Mutations in TWIST1 are implicated in Saethre-Chotzen syndrome, characterized by coronal synostosis, brachycephaly, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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