Thermo Fisher Scientific CDKN1B Monoclonal Antibody (10B7)
CDKN1B 단백질을 인식하는 Mouse 모노클로날 항체로, Western blot 및 IHC(P) 검증 완료. 세포 주기 조절 연구에 적합하며, 고순도 Protein G 정제 및 안정적 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:25
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10B7 |
| Immunogen | This CDKN1B antibody is generated from a mouse immunized with CDKN1B recombinant protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFβ-induced G1 arrest.
It is an important regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes.
p27 Kip1 forms a complex with cyclin D-CDK4 complexes and is involved in assembly, stability, and modulation of CCND1-CDK4 complex activation.
Depending on its phosphorylation state and stoichiometry, p27 Kip1 acts either as an inhibitor or activator of cyclin D-CDK4 complexes.
It has a molecular weight of approximately 27 kDa, and its degradation—triggered by CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes—is required for the transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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