Thermo Fisher Scientific GRAMD1A Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human GRAMD1A. Recombinant protein control fragment (Product #RP-90008). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GRAMD1A,uniProtId:Q96CP6-1,ncbiNodeId:9606,antigenRange:225-339,antigenLength:724,antigenImageFileName:PA5-52617_GRAMD1A_Q96CP6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52617_GRAMD1A_Q96CP6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2642193

Product Specific Information

Immunogen sequence: SEDEDYVSPL QLNGLGTPKE VGDVIALSDI TSSGAADRSQ EPSPVGSRRG HVTPNLSRAS SDADHGAEED KEEQVDSQPD ASSSQTVTPV AEPPSTEPTQ PDGPTTLGPL DLLPS

Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 85%.

Target Information

Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER. Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol. In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain. At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER. May play a role in tumor progression. Plays a role in autophagy regulation and is required for biogenesis of the autophagosome (PubMed:31222192). This function in autophagy requires its cholesterol-transfer activity (PubMed:31222192). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.