Thermo Fisher Scientific p19ARF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

1:50-1:100

View 1 publication 1 publication

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p19ARF,uniProtId:Q64364-1,ncbiNodeId:10090,antigenRange:50-150,antigenLength:169,antigenImageFileName:PA1-30670_p19ARF_Q64364-1_Rabbit.svg,antigenImageFileNamePDP:PA1-30670_p19ARF_Q64364-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/tris phosphate, pH 7.0-8.0

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1956784

Product Specific Information

PA1-30670 recognizes p19ARF, Uniprot ID Q64364.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.