Thermo Fisher Scientific ARC Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 191-208 of Human ARC.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1956181

Product Specific Information

Suggested positive control is Hela whole cell lysate.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.