Thermo Fisher Scientific CRYAB Monoclonal Antibody (OTI6G5), TrueMAB
인간 CRYAB 단백질을 인식하는 Mouse IgG2b 단일클론 항체입니다. Western blot, IHC, Flow cytometry, IP 등 다양한 응용에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6G5 |
| Immunogen | Full-length human recombinant CRYAB protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Lens proteins are composed primarily of crystallins (~95%), which are also found in vertebrate skeletal muscle.
Crystallins maintain the lens refractive index and include three major classes: alpha, beta, and gamma.
Alpha-crystallin, the largest class, consists of alpha-A and alpha-B subunits.
Alpha-B crystallin plays a chaperone-like role, preventing aggregate formation that could lead to cataracts.
Phosphorylation of alpha-crystallin occurs in response to cellular stress and may contribute to structural control and protein maintenance.
Alpha-B crystallin accumulation is associated with Alexander’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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