Thermo Fisher Scientific CoA Synthase Recombinant Rabbit Monoclonal Antibody (JE58-56)
Recombinant rabbit monoclonal antibody targeting human CoA Synthase (COASY). Validated for Western blot and flow cytometry. High purity (Protein A purified, 1 mg/mL). Suitable for research use in mitochondrial and cytoplasmic localization studies.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE58-56 |
| Immunogen | Synthetic peptide within Human COASY aa 510–564/564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092893 |
Product Specific Information
- Positive control: HepG2 cell lysates
- Predicted band size: 62 kDa
- Subcellular location: Mitochondrion matrix, Cytoplasm
Target Information
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes.
COASY is a bifunctional enzyme that catalyzes the two last steps in CoA synthesis.
These activities are performed by two separate enzymes, phosphopantetheine adenylyltransferase and dephospho-CoA kinase, in prokaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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