Thermo Fisher Scientific ERK1 Monoclonal Antibody (12D11)
ERK1 단백질을 특이적으로 인식하는 mouse monoclonal antibody (Clone 12D11). Western blot, IHC, ICC/IF, ChIP 등에 사용 가능하며 ERK2와 교차 반응하지 않음. Human, Mouse, Non-human primate 시료에 반응. Protein A 정제, PBS+BSA buffer에 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| ChIP assay (ChIP) | 1–3 µL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12D11 |
| Immunogen | Full-length human recombinant ERK1 protein expressed in bacteria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979495 |
Product Specific Information
MA1-13041 detects ERK1 from human, mouse, and monkey samples.
This antibody does not cross-react with ERK2.
It has been successfully used in Western blot, ChIP, immunofluorescence, and immunohistochemistry procedures.
By Western blot, this antibody detects a 44 kDa protein corresponding to ERK1.
The antigen is a recombinant protein corresponding to the full-length sequence of human ERK1.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway.
ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
Various stimuli such as growth factors, cytokines, viral infection, and ligands for G protein-coupled receptors activate ERK1/ERK2 pathways.
Functionally, ERK1 participates in signaling cascades that control proliferation, differentiation, and cell cycle progression.
Activated ERK1 translocates to the nucleus to phosphorylate nuclear targets.
Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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