Thermo Fisher Scientific ERK1 Monoclonal Antibody (12D11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ChIP assay (ChIP)

1-3 µL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

12D11

Immunogen

Full-length human recombinant protein expressed in bacteria. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERK1,uniProtId:P27361-1,ncbiNodeId:9606,antigenRange:1-379,antigenLength:379,antigenImageFileName:MA1-13041_ERK1_P27361-1_House_mouse.svg,antigenImageFileNamePDP:MA1-13041_ERK1_P27361-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979495

Product Specific Information

MA1-13041 detects ERK1 from human, mouse, and monkey samples. MA1-13041 does not cross react with ERK2.

MA1-13041 has been successfully used in Western blot, ChIP, immunofluoresence, and immunohistochemistry procedures. By Western blot, this antibody detects a 44 kDa protein corresponding to ERK1.

The MA1-13041 antigen is a recombinant protein corresponding to the full-length sequence of human ERK1.

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.