Thermo Fisher Scientific Phospho-Src (Tyr529) Polyclonal Antibody
Src 단백질의 Tyr529 인산화 부위를 인식하는 rabbit polyclonal antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from mouse Src around the phosphorylation site of tyrosine 529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It plays a key role in growth and differentiation of eukaryotic cells, associates with various cell surface receptors for signal transduction, and participates in cytoskeletal organization, cell cycle regulation, and apoptosis.
In humans, SRC is located on chromosome 20. Its activity is regulated by phosphorylation at Tyr416 and Tyr527, which have opposing effects: phosphorylation at Tyr416 activates the enzyme, while phosphorylation at Tyr527 inhibits it. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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