Thermo Fisher Scientific PAX2 Polyclonal Antibody, FITC
FITC 결합형 PAX2 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot, IHC, ELISA, IP 등 다양한 응용 가능. 합성 펩타이드(AA 1-50) 면역원 사용. 액상 형태, 친화 크로마토그래피 정제, -20°C 암저장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 1–50 on human PAX2 protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PAX genes are a family of transcription factors active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box, enabling them to execute genetic programs.
Several Pax genes are associated with developmental mutations, such as:
- PAX3: Waardenburg syndrome
- PAX6: Aniridia
- PAX2: Wilms tumor
During embryogenesis, PAX2 is expressed in the developing kidney, particularly in condensing metanephric mesenchyme and early epithelial structures. Expression decreases as tubular epithelium matures but persists in undifferentiated epithelium of Wilms tumors, correlating with poorly differentiated epithelial proliferation.
The Wilms tumor suppressor protein WT1 coincides with down-regulation of PAX2, suggesting WT1 directly represses PAX2 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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