Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–737
• Brilliant Violet 421, 605, 650
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm, suitable for use with a 405 nm violet laser. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific interactions.

Light Sensitivity:
Protect from light; the tandem dye is sensitive to photo-induced oxidation.

Fixation Compatibility:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49) or
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  for up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD44 is a 100 kDa type I transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells. It serves as a receptor for hyaluronic acid (HA), mediating cell-cell interactions, adhesion, and migration. CD44 is involved in lymphocyte activation, recirculation, and homing. Expression may increase in certain carcinomas, potentially linked to metastasis. Multiple isoforms exist due to alternative splicing, contributing to functional diversity. CD44 dysfunction is associated with diseases such as superficial keratitis and lichen sclerosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지