Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 610-30S, 660-40S
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) expressed in CD45.1 mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes (≤1.0 µg/test). A test is defined as the amount of antibody that stains a cell sample in 100 µL volume (10⁵–10⁸ cells/test).
• Optimization: Antibody titration recommended for best performance.

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is designed for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple Brilliant Violet™ or polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42), or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific interactions.

Handling Notes:

• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
• Compatibility: BV711 is not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser.

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising about 10% of surface proteins in lymphocytes. It regulates Src-family kinases, influencing lymphocyte development and antigen signaling.
CD45 exists as multiple isoforms from alternative splicing, differing in extracellular domains. Isoforms vary by cell type and physiological state. CD45 also modulates integrin-mediated signaling and contributes to apoptosis regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.