카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Norgen · Norgen Endotoxin Removal Kit (Midi) - For DNA
Norgen Endotoxin Removal Kit (Midi) - For DNA
SKU: 52200
For the rapid removal of endotoxins from previously purified DNA.
Features and Benefits
- Reduce endotoxin levels to 0.1 EU/µg DNA or less
- Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
- Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
- Fast and easy processing using a rapid spin-column format
These kits are designed for the rapid spin-column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts, and other contaminants are washed away. These kits reduce endotoxins to ≤0.1 EU/μg DNA, providing plasmid DNA ready for transfections or other endotoxin-sensitive applications. Typical DNA recovery is >90%.
Endotoxin Removal Kit Formats
Mini: Removes endotoxins from up to 25 µg DNA in ~20 minutes.
Midi: Removes endotoxins from up to 200 µg DNA in ~30 minutes.
Maxi: Removes endotoxins from up to 1 mg DNA in ~30 minutes.
About Endotoxins
Endotoxins (lipopolysaccharides) are components of Gram-negative bacterial membranes such as E. coli. They are released during plasmid purification and can reduce transfection efficiency. Removing endotoxins is essential for DNA used in sensitive downstream applications.
Supporting Data
Figure 1. Efficiently Remove Endotoxins
An input of 11 µg plasmid DNA (>8 EU/µg) was cleaned using Norgen’s Endotoxin Removal Kit. Endotoxin levels were reduced to ≤0.1 EU/µg with high DNA recovery.
그래프 요약:
Average EU/µg Plasmid
Before: 약 8
After: 거의 0
Figure 2. High DNA Recovery
DNA recovery was >90% after endotoxin removal using the Mini kit.
| 항목 | % Recovery (대략적) |
|---|---|
| Input DNA | 약 100% |
| Recovered DNA | 약 90% |
Kit Specifications
| 항목 | 내용 |
|---|---|
| Maximum DNA Input | 200 µg |
| Final Endotoxin Level | ≤ 0.1 EU/μg DNA |
| Size of DNA Purified | Up to 13,000 bp |
| Maximum DNA Volume Input | 0.75 mL |
| Average Recovery | >90% (~80% in First Elution) |
| Time to Complete 10 Purifications | 30 minutes |
Storage Conditions and Product Stability
All solutions should be tightly sealed and stored at room temperature.
Stable for 2 years from shipment date.
| Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
|---|---|---|---|
| Buffer SK | 15 mL | 60 mL | 60 mL |
| Wash Solution H | 18 mL | 18 mL | 18 mL |
| Elution Buffer I | 6 mL | 12 mL | 12 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
| Precipitation Solution | - | 1.5 mL | 1.5 mL |
| Spin Columns (assembled with Collection Tubes) | - | 10 | 4 |
| Spin Columns | 25 | - | - |
| Collection Tubes | 25 | - | - |
| Elution Tubes (1.7 mL) | 25 | - | - |
| Elution Tubes (15 mL) | - | 10 | - |
| Elution Tubes (50 mL) | - | - | 4 |
| Product Insert | 1 | 1 | 1 |
Documentation
Protocols
- (22700) Endotoxin Removal Kit (Mini) - Protocol (25 prep)
- (52200) Endotoxin Removal Kit (Midi) - Protocol (10 prep)
- (21900) Endotoxin Removal Kit (Maxi) - Protocol (4 preps)
Safety Data Sheet (SDS)
Flyer
FAQs
Why do I have poor DNA recovery?
- DNA did not bind properly to the column (check Buffer SK for precipitates)
- Incorrect ethanol amount added to Wash Solution H
- Incomplete removal of Wash Solution H (spin adequately)
- Incorrect Buffer SK volume used
Why is purified DNA not performing well?
- Wash Solution H not used properly
- Incorrect or non-optimized Elution Buffer used
- Elution buffer may contain interfering components (e.g., salts, detergents)
Why are endotoxin levels slightly higher than 0.1 EU/µg DNA?
- Different elution buffer used
- Extremely high initial endotoxin levels (repeat purification recommended)
Citations
| Title | Citation | Authors |
|---|---|---|
| Rational design and construction of multi-copy biomanufacturing islands in mammalian cells | Nucleic Acids Research, 2022 | Altamura R, Doshi J, Benenson Y |
| Precision multidimensional assay for high-throughput microRNA drug discovery | Nature Communications, 2016 | Benjamin Haefliger, Laura Prochazka, Bartolomeo Angelici, Yaakov Benenson |
| Transplantation of prokaryotic two-component signaling pathways into mammalian cells | PNAS, 2014 | J Hansen, E Mailand, KK Swaminathan, J Schreiber, B Angelici, Y Benenson |
Related Products
Genomic DNA Isolation Kit
- Cat. 24700, 50 Preps
- Components include Digestion Buffer A (18 mL), Wash Solution A (18 mL), Buffer SK (30 mL)
- Store at room temperature
| 구성품 | 용량 | 보관 조건 |
|---|---|---|
| Digestion Buffer A | 18 mL | Room temperature |
| Wash Solution A | 18 mL | Room temperature |
| Buffer SK | 30 mL | Room temperature |
Fungi/Yeast Genomic DNA Isolation Kit
Phage DNA Isolation Kit
제품 이미지
Norgen 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.