Thermo Fisher Scientific NENF Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NENF. Recombinant protein control fragment (Product #RP-94160). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NENF,uniProtId:Q9UMX5-1,ncbiNodeId:9606,antigenRange:45-104,antigenLength:172,antigenImageFileName:PA5-55410_NENF_Q9UMX5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55410_NENF_Q9UMX5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644613

Product Specific Information

Immunogen sequence: RLFTEEELAR YGGEEEDQPI YLAVKGVVFD VTSGKEFYGR GAPYNALTGK DSTRGVAKMS

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 95%.

Target Information

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.